ĚÇĐÄÍřŇł°ć

18 December 2023

Scientists, including Linda Bojmar as first author, have introduced a comprehensive protocol for the cross-platform characterization of extracellular vesicles and particles (EVPs) in both humans and mice.

The protocol aims to provide a standardized approach for analyzing EVPs, crucial for unraveling their functions and exploring biomarker potential.

Key Highlights of the Protocol

Cross-Validation Across Platforms: The protocol emphasizes the cross-validation of isolated EVPs across different platforms, ensuring robust and consistent results.

Multifaceted Analysis: Researchers can now employ various techniques for EVP analysis, including nanoparticle tracking analysis and transmission electron microscopy, allowing for the examination of EVP size, number, and morphology.

Protein Cargo Identification: The protocol introduces a novel approach to EVP protein cargo identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with bioinformatic analysis.

Validation Techniques: To verify the presence of specific EVP proteins, the protocol advocates for single-particle analysis, western blot analysis, and ExoELISA, providing a comprehensive validation framework.

The significance of this protocol lies in its ability to facilitate EVP cross-validation across different platforms, enhancing the reliability of research outcomes. By offering a standardized methodology, scientists can now delve deeper into the intricate world of extracellular vesicles and particles, paving the way for breakthroughs in understanding their roles and potential as biomarkers.

This protocol is expected to catalyze advancements in the study of EVPs, opening new avenues for therapeutic development and diagnostic applications.

Contact

Latest news from LiU

Woman at office.

Biogenic carbon dioxide could become a key resource as biogas expands

During the production and upgrading of biogas, carbon dioxide is released, a greenhouse gas that affects the climate. However, research at ĚÇĐÄÍřŇł°ć shows that this carbon dioxide has several uses and could become an important resource.

A man wearing glasses standing in front of a red wall.

Moral economy perspectives through history

When a crisis arises, a humanitarian urge to help others is often awakened. But what happens when emotions rule and knowledge is lacking? Norbert Götz, professor of modern history, has researched the conditions of humanitarian aid.

A couple of men standing next to each other in front of a blackboard.

Scientists mimic heart muscle cells with conductive plastic

For the first time, scientists have succeeded in artificially mimicking the ion signalling of heart muscle cells. To succeed, researchers at LiU have used organic electronics. The findings open up for new types of implants and sensors.