ÌÇÐÄÍøÒ³°æ

8-capillary platform

Applications:

• Sanger Sequencing
• Microsatellites

Old technology, but fast and inexpensive to use with simple setup, for mutation confirmation and microsatellite/STR analysis.

With MiSeq, you can:

• Multiplex up to 96 samples per run for greater efficiency.
• Get accurate bi-directional amplicon sequencing.
• Generate complete de novo assemblies.
• Produce 2 x 300 paired-end reads and up to 15 GB of data in a single run.

With NextSeq 550, you can perform:

• Targeted sequencing (amplicon-based, gene panel)
• Transcriptome sequencing (total RNA-Seq, mRNA-Seq, gene expression profiling)
• Arrays scanning

With NextSeq 200, you can perform:

• Targeted sequencing (amplicon-based, gene panel)
• Small Whole Genome Sequencing (WGS)
• Whole exome sequencing (WES)
• Transcriptome sequencing (total RNA-Seq, mRNA-Seq, small RNA-Seq, single cell RNA-Seq)

To choose between the different available sequencing platforms, we recommend using the following link: 

The PromethION 2 Integrated (P2i) is a compact benchtop device for high-output nanopore sequencing. It can run up to two PromethION flow cells simultaneously, allowing for flexible and efficient sequencing. The P2i includes powerful integrated compute capabilities, featuring an NVIDIA Ampere GPU, which enables real-time data processing and basecalling.

This device can generate up to 290 gigabases of data from a single flow cell. It also features a high-resolution touchscreen display and robust storage and memory specifications, making it a versatile tool for various sequencing applications.

Capacity: 96-well fast PCR plates. Equipped with 5 emission filters for: SYBR® Green I, FAM™, VIC™, JOE™, NED™, TAMRA™, ROX™, Texas Red®, Cy3™, Cy5™.

Applications:

• absolut quantification
• relative quantification
• allele discrimination +/- detection.

The QuantStudioâ„¢ 7 Flex system for 96-well Fast, 384-well, or TaqMan® array card block. 

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications.

2720 Thermal Cycler from Applied Biosystems. 96 well format.

C1000 Touch Thermal Cycler from BioRad. 96 well format with gradient possibilities

The Affymetrix instrument for microarray analysis is an integrated platform containing scanner, oven, fluidics station.

The microarray platform allows whole genome gene expression and miRNA expression analysis for a wide variety of organisms (Human, Mouse, Zebrafish etc.)

Click for more 

The Maxwell® Rapid Sample Concentrator (RSC) Instrument is a platform for automated purification of nucleic acid from a range of sample types. It can process up to 16 samples simultaneously, using prefilled cartridges and preprogrammed methods. DNA or RNA extraction in 25–60 minutes, depending upon sample type.

QIAcube Connect performs fully automated purification of nucleic acids and proteins in molecular biology applications. It automates over 80 QIAGEN kits with over 140 standard protocols for DNA, RNA and protein sample processing.

The Qiagen Tissue Lyser II is used for high-throughput disruption of a wide range of biological samples (animal and plant tissues, bacteria and yeast).

You must buy your own QIAGEN kits and Beads.

The Bioruptor® Pico is a versatile sonicator for DNA, RNA, chromatin, and protein shearing with high precision and reproducibility. It allows parallel processing with built-in cooling to protect samples.

Capillary electrophoresis for detection of large fragment DNA and libraries for NGS.

Analysis kits available at Cellkärnan for:

• Standard Sensitivity NGS Fragment (1 bp–6,000 bp)
• High Sensitivity NGS Fragment (1 bp–6,000 bp)
• Standard Sensitivity Large Fragment (50 bp–20,000 bp)

The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of DNA/RNA. Agilent RNA 6000 nano kit available at Cellkärnan.

Quantus is a sensitive single tube fluorometer for fluorescence-based quantitation of DNA and RNA. It allows the quantification of low-level DNA/RNA samples accurately. DNA one kit and QuantiFlour RNA system are available at Cellkärnan.

Vacuum concentrator for removing solvents and concentrating samples while maintaining sample integrity.

The 4150 TapeStation system is a low-throughput automated electrophoresis platform for nucleic acid sample quality control. It allows a quick, easy, and reliable analysis of DNA and RNA samples for up to 16 samples per run. D100 and HS RNA kits are available at Cellkärnan.

NanoDrop™ One is a part of Thermo Scientific™. Quantify and qualify DNA, RNA, and protein samples in seconds with only 1–2 μL, and obtain full-spectral data before you decide to use samples in downstream applications.